TABLE 1.

Synergistic activities in vitro of phenothiazines and omeprazole with antibiotics against B. pseudomallei KHW

Antibiotic	EPI	MIC of antibiotic (μg/ml) at indicated concn of phenothiazine or omeprazole (μM)a:										Maximum decrease in MIC (n-fold)
		0	1,000	500	250	125	62.5	31.3	15.6	7.8	3.9
Aminoglycoside
Streptomycin	PCPZ	1,024	256	256	512	1,024	1,024	1,024	1,024	1,024	1,024	4
	CPZ	1,024	<0.25	<0.25b	1,024	1,024	1,024	1,024	1,024	1,024	1,024	>4,000
	PMZ	1,024	0.5	516	1,024	1,024	1,024	1,024	1,024	1,024	1,024	>2,000
	OPZ	1,024	<0.25b	1,024	1,024	1,024	1,024	1,024	1,024	1,024	1,024	>4,000
Spectinomycin	PCPZ	256	<0.25	<0.25b	32	32	128	256	256	256	256	>1,000
	CPZ	256	<0.25	32	128	256	256	256	256	256	256	>1,000
	PMZ	256	<0.25b	64	64	256	256	256	256	256	256	>1,000
	OPZ	256	32	64	64	256	256	256	256	256	256	8
Macrolide
ERY	PCPZ	128	8	8	8	32	32	64	64	128	128	16
	CPZ	128	<0.25	8	32	128	128	128	128	128	128	>500
	PMZ	128	<0.25	32	64	128	128	128	128	128	128	>500
	OPZ	128	<0.25	128	128	128	128	128	128	128	128	>500
Oleandomycin	PCPZ	>2,000	64	64	128	256	256	512	512	>2,000	>2,000	>30
	CPZ	>2,000	<0.25	128	256	512	1,024	>2,000	>2,000	>2,000	>2,000	>8,000
	PMZ	>2,000	0.5	256	>2,000	>2,000	>2,000	>2,000	>2,000	>2,000	>2,000	>4,000
	OPZ	>2,000	<0.25	>2,000	>2,000	>2,000	>2,000	>2,000	>2,000	>2,000	>2,000	>8,000
AZM	PCPZ	32	<0.25	<0.25	<0.25b	16	32	32	32	32	32	>128
	CPZ	32	<0.25	8	16	16	32	32	32	32	32	>128
	PMZ	32	<0.25	16	32	32	32	32	32	32	32	>128
	OPZ	32	16	16	32	32	32	32	32	32	32	2
β-Lactam
AMC	PCPZ	16	<0.25	<0.25	<0.25b	16	16	16	16	16	16	>64
	CPZ	16	0.5	16	16	16	16	16	16	16	16	32
	PMZ	16	0.5	16	16	16	16	16	16	16	16	32
	OPZ	16	16	16	16	16	16	16	16	16	16	1
Fluoroquinolone
LVX	PCPZ	2	<0.25	<0.25b	2	2	2	2	2	2	2	>8
	CPZ	2	<0.25	<0.25	1	2	2	2	2	2	2	>8
	PMZ	2	<0.25	1	2	2	2	2	2	2	2	>8
	OPZ	2	1	2	2	2	2	2	2	2	2	2

^aValues in boldface denote synergistic interaction between each respective phenothiazine, or omeprazole, and antibiotic.

^bDenotes the lowest concentration of antibiotic used in this assay.