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SDS-PAGE of the purification procedure of APH(3′)-IIb. (A) Molecular size markers (bottom to top) of 10, 15, 20, 25, 37, 50, 75, and 100 kDa; (B) crude extract; (C) protein preparation after Q-Sepharose column elution; (D) purified APH(3′)-IIb after neomycin affinity column elution.
