TABLE 2.

MICs of several aminoglycosides against P. aeruginosa, the E. coli background strain, and its engineered variant^a

Compound	MIC (μg/ml)
	P. aeruginosa	E. coli	E. coli R
Kanamycin A	256	12	768
Neomycin B	128	12	96
Neamine	512	96	1,536
Ribostamycin	384	12	768
Amikacin	3	12	12
Tobramycin	1	6	6
Gentamicin	2	6	6

^aP. aeruginosa ATCC 27853, E. coli BL21(DE3) CodonPlus-RP, and E. coli BL21(DE3) CodonPlus-RP/pET11d-aph(3′)-IIb expressing the APH(3')-IIb enzyme (E. coli R).