TABLE 3.
Peptides | λmax buffer (nm) | Blue shift (nm)
|
KSV (M−1)a
|
|||
---|---|---|---|---|---|---|
POPG | POPC | Buffer | POPG | POPC | ||
RW-NH2 | 350 | 10 | 6.5 | 15 | 2.5 | 2.3 |
(RW)2-NH2 | 350 | 11 | 6.0 | 14 | 2.7 | 2.5 |
(RW)3-NH2 | 349 | 11 | 6.0 | 18 | 1.8 | 2.8 |
(RW)4-NH2 | 350.5 | 10 | 6.0 | 17 | 2.3 | 5.8 |
(RW)5-NH2 | 348 | 11 | 8.5 | 25 | 2.7 | 5.3 |
Stern-Vollmer constants, KSV (M−1) were determined from the Stern-Vollmer equation F0/F = 1 + KSV(Q), where Q is the concentration of quencher (acrylamide). Concentrations of the quencher varied from 0.01 to 0.40 M.