TABLE 2.
Phenotypic drug susceptibility of HIV-2 and HIV-1 to PIs
| Viral isolate (no. of isolates) | PI mutation(s) | Mean IC50 (μM) ± SDa
|
||||
|---|---|---|---|---|---|---|
| APV | IDV | NFV | TPV | LPV | ||
| HIV-2 WT (3) | None | 0.440 ± 0.369 | 0.016 ± 0.011 | 0.064 ± 0.074 | 0.160 ± 0.075 | 0.004 ± 0.003 |
| I82L | 0.528 ± 0.300 (1.2) | 0.064 ± 0.021 (4) | 0.106 ± 0.050 (1.7) | 4.436 ± 2.867 (27.7) | 0.075 ± 0.074 (18.8) | |
| I82F | 0.965 ± 0.166 (2.2) | 0.544 ± 0.021 (34) | 0.244 ± 0.063 (4) | 0.306 ± 0.069 (2) | 0.145 ± 0.050 (36.3) | |
| V62A, L99F | 0.098 ± 0.071 | 0.143 ± 0.022 (9) | 0.961 ± 0.228 (15) | 0.524 ± 0.302 (3.3) | 0.496 ± 0.031 (124) | |
| I50V | >10 (>22.7) | 0.072 ± 0.001 (4.5) | 0.132 ± 0.013 (2.1) | 0.316 ± 0.014 (2) | 0.203 ± 0.139 (50.8) | |
| I54M | >10 (>22.7) | 0.236 ± 0.051 (15) | 0.624 ± 0.0.368 (10) | 1.106 ± 0.468 (7) | 0.481 ± 0.275 (120.3) | |
| I54M, L90M | >10 (>22.7) | 1.112 ± 0.447 (70) | 2.564 ± 0.390 (40) | 0.145 ± 0.001 | 0.524 ± 0.004 (131) | |
| I54M, I84V | >10 (>22.7) | 0.388 ± 0.011 (24.3) | 1.747 ± 0.074 (27.3) | 2.028 ± 0.144 (12.7) | 0.375 ± 0.006 (93.8) | |
| I54M, L99F | >10 (>22.7) | 0.825 ± 0.377 (52) | 2.241 ± 0.303 (35) | 2.908 ± 0.544 (18.2) | 0.414 ± 0.010 (103.5) | |
| HIV-1 WT (1) | None | 0.026 ± 0.003 | 0.027 ± 0.001 | 0.014 ± 0.007 | 0.092 ± 0.013 | 0.002 ± 0.001 |
| V32I, M46L | 0.116 ± 0.012 (4.5) | 0.045 ± 0.005 (1.7) | 0.031 ± 0.004 (2.2) | 0.042 ± 0.003 | 0.001 ± 0.001 | |
| D30N, M46I, V77I | 0.019 ± 0.004 | 0.025 ± 0.006 | 0.677 ± 0.221 (48.4) | 0.001 ± 0.001 | 0.002 ± 0.001 | |
| M36I/M, V82T | 0.003 ± 0.002 | 0.014 ± 0.003 | 0.010 ± 0.001 | 0.192 ± 0.013 (2.1) | 0.007 ± 0.002 (3.5) | |
a
The values represent the means of at least two independent experiments, each performed in duplicate. The values in parentheses are the fold resistance compared to that of wild-type (WT) HIV-2 or HIV-1. The mean IC50s of the HIV-2 wild-type isolates were 0.440 ± 0.369 μM for APV, 0.016 ± 0.011 μM for IDV, 0.064 ± 0.074 μM for NFV, 0.160 ± 0.075 μM for TPV, and 0.004 ± 0.003 μM for LPV, while those of HIV-1 were 0.026 ± 0.003 for APV, 0.027 ± 0.001 for IDV, 0.014 ± 0.007 for NFV, 0.092 ± 0.013 for TPV, and 0.002 ± 0.001 for LPV. The HIV-2 isolates included CBL-20, CBL-23, and MVP-15132; the HIV-1 isolate was 5512.