TABLE 3.
Antimicrobial susceptibility phenotypes of deletion and replacement mutants of serovar Typhimurium S21 and wild-type serovar Typhimurium strains CS1, CS3, CS9, CHS14, CHS18, and CHS38a
| Strain | Amino acid substitution(s)b
|
Quinolone and fluoroquinolone MICs (μg/ml)
|
Other antimicrobial MICs (μg/ml)
|
|||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GyrA | ParC | NAL | CIP | DAN | DIF | ENR | GAT | LEV | ORB | SAR | AMO | AMP | CEP | CHL | COT | FOX | KAN | TET | TIO | |
| S21 | * | * | 4 | <0.015 | 0.03 | 0.25 | 0.06 | 0.03 | 0.03 | 0.12 | 0.03 | <1/05 | 2 | <2 | 8 | 0.25/4.75 | 4 | <8 | <4 | 0.5 |
| S21 gyrA+ | * | * | 4 | <0.015 | 0.03 | 0.25 | 0.06 | 0.03 | 0.03 | 0.12 | 0.03 | <1/05 | 2 | <2 | 8 | 0.25/4.75 | 4 | <8 | <4 | 0.5 |
| S21 acrAB::Kan | * | * | 1 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <1/0.5 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21 tolC::Kan | * | * | 1 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <1/0.5 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-1 | S83F | * | 256 | 0.25 | 0.5 | 4 | 0.5 | 0.5 | 0.5 | 2 | 1 | 16/8 | >32 | 8 | >32 | 0.25/4.75 | 2 | <8 | 32 | 0.5 |
| S21-1 gyrA+ | * | * | 4 | 0.015 | 0.03 | 0.25 | 0.06 | 0.03 | 0.03 | 0.12 | 0.03 | 16/8 | >32 | 8 | >32 | 0.25/4.75 | 2 | <8 | 32 | 0.5 |
| S21-1 acrAB::Kan | S83F | * | 32 | 0.06 | 0.06 | 0.25 | 0.06 | 0.03 | 0.06 | 0.12 | 0.12 | 16/8 | >32 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-1 tolC::Kan | S83F | * | 32 | 0.06 | 0.06 | 0.25 | 0.06 | 0.03 | 0.06 | 0.12 | 0.12 | 16/8 | >32 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-2 | S83F | S80I | >256 | 4 | 4 | >16 | 8 | 1 | 1 | 16 | 8 | 16/8 | >32 | >32 | >32 | 2/38 | >16 | <8 | >32 | 4 |
| S21-2 gyrA+ | * | S80I | 64 | 0.25 | 1 | 2 | 1 | 0.25 | 0.25 | 2 | 0.25 | 16/8 | >32 | >32 | >32 | 2/38 | >16 | <8 | >32 | 4 |
| S21-2 acrAB::Kan | S83F | S80I | 64 | 0.12 | 0.12 | 0.25 | 0.12 | 0.06 | 0.12 | 0.25 | 0.5 | 16/8 | >32 | 8 | <2 | <0.12/2.38 | 2 | >64 | <4 | <0.12 |
| S21-2 tolC::Kan | S83F | S80I | 64 | 0.12 | 0.12 | 0.25 | 0.12 | 0.06 | 0.06 | 0.25 | 0.5 | 16/8 | >32 | 8 | <2 | <0.12/2.38 | 2 | >64 | <4 | <0.12 |
| S21-3 | S83F | S80I | >256 | 32 | >16 | >16 | >16 | 8 | >16 | 16 | >16 | 16/8 | >32 | >32 | >32 | >4/76 | >16 | <8 | >32 | 4 |
| S21-3 gyrA+ | * | S80I | >256 | 2 | 2 | 4 | 2 | 1 | 1 | 2 | 1 | 16/8 | >32 | >32 | >32 | >4/76 | >16 | <8 | >32 | 4 |
| S21-3 acrAB::Kan | S83F | S80I | 64 | 0.25 | 0.25 | 0.5 | 0.25 | 0.06 | 0.12 | 0.5 | 0.5 | 16/8 | >32 | 8 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-3tolC::Kan | S83F | S80I | 32 | 0.25 | 0.25 | 0.5 | 0.12 | 0.06 | 0.12 | 0.5 | 0.5 | 16/8 | >32 | 8 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-4 | S83F | S80I | >256 | 256 | >16 | >16 | >16 | 16 | >16 | 16 | >16 | 16/8 | >32 | >32 | >32 | >4/76 | >16 | <8 | >32 | 4 |
| S21-4 gyrA+ | * | S80I | >256 | 4 | 4 | 4 | 4 | 2 | 2 | 8 | 2 | 16/8 | >32 | >32 | >32 | >4/76 | >16 | <8 | >32 | 4 |
| S21-4 acrAB::Kan | S83F | S80I | >256 | 0.25 | 0.25 | 1 | 0.12 | 0.06 | 0.25 | 0.12 | 0.5 | 16/8 | >32 | 8 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21-4 tolC::kanC | S83F | S80I | >256 | 0.25 | 0.25 | 1 | 0.12 | 0.06 | 0.25 | 0.12 | 0.5 | 16/8 | >32 | 8 | 32 | <0.12/2.38 | 2 | >64 | 8 | <0.12 |
| S21 | * | * | 4 | <0.015 | 0.03 | 0.25 | 0.06 | 0.03 | 0.03 | 0.12 | 0.03 | <1/05 | 2 | <2 | 8 | 0.25/4.75 | 4 | <8 | <4 | 0.5 |
| S21 gyrA+ | * | * | 4 | <0.015 | 0.03 | 0.25 | 0.06 | 0.03 | 0.03 | 0.12 | 0.03 | <1/05 | 2 | <2 | 8 | 0.25/4.75 | 4 | <8 | <4 | 0.5 |
| S21 acrAB::Kan | * | * | 1 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <1/0.5 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| S21 tolC::Kan | * | * | 1 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <1/0.5 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | <4 | <0.12 |
| CS1 | S83F, D87N | S80I | >256 | 16 | >16 | >16 | 16 | 4 | 8 | >16 | 16 | 32/16 | >32 | 16 | >32 | >4/76 | 1 | <8 | >32 | 1 |
| CS1 gyrA+ | S83F, D87N | S80I | 4 | 0.03 | 0.06 | 0.5 | 0.06 | 0.06 | 0.06 | 0.25 | 0.06 | 32/16 | >32 | 8 | >32 | >4/76 | 2 | >64 | >32 | 1 |
| CS1 acrAB::Kan | S83F, D87N | S80I | >256 | 4 | 4 | 16 | 4 | 0.5 | 2 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | >32 | 0.5 |
| CS1 tolC::Kan | S83F, D87N | S80I | 256 | 2 | 4 | 4 | 4 | 0.5 | 1 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | 32 | 0.5 |
| CS3 | S83F, D87N | S80I | >256 | 16 | >16 | >16 | 16 | 4 | 8 | >16 | 16 | 32/16 | >32 | 16 | >32 | >4/76 | 1 | <8 | >32 | 1 |
| CS3 gyrA+ | S83F, D87N | S80I | 4 | 0.03 | 0.06 | 0.5 | 0.06 | 0.06 | 0.06 | 0.25 | 0.06 | 32/16 | >32 | 8 | >32 | >4/76 | 2 | >64 | >32 | 1 |
| CS3 acrAB::Kan | S83F, D87N | S80I | >256 | 4 | 4 | 16 | 4 | 0.5 | 2 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | >32 | 0.5 |
| CS3 tolC::Kan | S83F, D87N | S80I | 256 | 2 | 4 | 4 | 4 | 0.5 | 1 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | 32 | 0.5 |
| CS9 | S83F, D87N | S80I | >256 | 16 | >16 | >16 | 16 | 4 | 8 | >16 | >16 | 32/16 | >32 | 16 | >32 | >4/76 | 1 | <8 | >32 | 1 |
| CS9 gyrA+ | S83F, D87N | S80I | 4 | 0.03 | 0.06 | 0.5 | 0.06 | 0.06 | 0.06 | 0.25 | 0.03 | 32/16 | >32 | 8 | >32 | >4/76 | 2 | >64 | >32 | 1 |
| CS9 acrAB::Kan | S83F, D87N | S80I | >256 | 4 | 4 | 16 | 4 | 0.5 | 2 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | >32 | 0.5 |
| CS9 tolC::Kan | S83F, D87N | S80I | 256 | 2 | 4 | 4 | 4 | 0.5 | 1 | 4 | 4 | 32/16 | >32 | 8 | >32 | 2/38 | 1 | >64 | 32 | 0.5 |
| CHS14 | D87G | * | 128 | 0.12 | 0.25 | 1 | 1 | 0.25 | 0.25 | 1 | 0.25 | 2/1 | <1 | 4 | 8 | 0.25/4.75 | 4 | <8 | >32 | 1 |
| CHS14 gyrA+ | D87G | * | 4 | <0.015 | 0.03 | 0.5 | 0.06 | 0.03 | 0.06 | 0.12 | 0.03 | 2/1 | <1 | <2 | 8 | 0.25/4.75 | 2 | >64 | >32 | 1 |
| CHS14 acrAB::Kan | D87G | * | 8 | <0.015 | 0.03 | 0.03 | 0.03 | <0.015 | 0.03 | 0.03 | <0.015 | 2/1 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | >32 | <0.12 |
| CHS14 tolC::Kan | D87G | * | 16 | <0.015 | 0.03 | 0.03 | <0.015 | <0.015 | <0.015 | 0.03 | <0.015 | 2/1 | <1 | <2 | <2 | <0.12/2.38 | 1 | >64 | >32 | <0.12 |
| CHS18 | S83F | * | 256 | 0.12 | 0.5 | 2 | 0.5 | 0.25 | 0.25 | 1 | 0.25 | 1/0.5 | 2 | 4 | 8 | 0.12/2.38 | 2 | 8 | 4 | 1 |
| CHS18 gyrA+ | S83F | * | 32 | 0.06 | 0.06 | 0.25 | 0.12 | 0.06 | 0.06 | 0.12 | 0.06 | 1/0.5 | 2 | 4 | 4 | 0.12/2.38 | 2 | >64 | 4 | 1 |
| CHS18 acrAB::Kan | S83F | * | 16 | 0.03 | 0.03 | 1 | 0.25 | 0.12 | 0.03 | 0.5 | 0.03 | 1/0.5 | 1 | 2 | 2 | 0.12/2.38 | 1 | >64 | 4 | 0.12 |
| CHS18 tolC::Kan | S83F | * | 32 | 0.03 | 0.06 | 0.06 | 0.03 | 0.03 | 0.03 | 0.06 | 0.03 | 1/0.5 | 1 | 2 | 2 | 0.12/2.38 | 1 | >64 | 4 | 0.12 |
| CHS38 | D87G | * | 32 | 0.06 | 0.25 | 1 | 0.12 | 0.12 | 0.12 | 0.5 | 0.12 | 1/0.5 | >32 | 8 | 32 | >4/76 | 4 | 8 | 32 | 0.5 |
| CHS38 gyrA+ | D87G | * | 8 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.15 | <0.015 | 1/0.5 | >32 | 16 | 32 | >4/76 | 4 | >64 | 32 | 0.5 |
| CHS38 acrAB::Kan | D87G | * | 1 | 0.03 | 0.03 | 0.06 | 0.03 | <0.015 | 0.03 | 0.06 | 0.03 | 1/0.5 | >32 | 8 | 4 | >4/76 | 0.5 | >64 | 16 | 0.12 |
| CHS38 tolC::Kan | D87G | * | 0.5 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | <0.015 | 1/0.5 | >32 | 8 | 4 | >4/76 | 0.5 | >64 | 16 | 0.12 |
a
NAL, nalidixic acid; CIP, ciprofloxacin; DAN, danofloxacin; DIF, difloxacin; ENR, enofloxacin; GAT, gatifloxacin; LEV, levofloxacin; ORB, orbifloxacin; SAR, sarafloxacin; AMO, amoxicillin-clavulanate; AMP, ampicillin; CEP, cephalothin; CHL, chloramphenicol; COT, trimethoprim-sulfamethoxazole; FOX, cefoxitin; KAN, kanamycin; TET, tetracycline; and TIO, ceftiofur.
b
*, Wild-type allele (no mutation); S, serine; F, phenylalanine; I, isoleucine; D, aspartic acid; N, asparagine; G, glycine.