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Kinetics of GS-7340 and GS-9131 hydrolysis by CatA
| Compound | Km (μM) | kcat (s−1)a | kcat/Km (s−1 · M−1) |
|---|---|---|---|
| GS-7340 | 610 ± 190 | 1,777 ± 290 | 2.9 × 106 |
| GS-9131 | 363 ± 35 | 237 ± 12 | 0.6 × 106 |
kcat values were calculated from Vmax values with the assumption that all enzyme molecules are catalytically active. The amount of CatA in the sample was estimated based on the Western blot.
