FIG. 4.
Effect of amoxicillin (Clamoxyl) on the severity of the allergic reaction. (A) Experimental design of sensitization in amoxicillin-treated rats. Antibiotics were administered to the pups from PND7 to PND25. Sensitization was performed by subcutaneous injection of 50 μg OVA in Al(OH)3 at PND11. Challenge was performed by intragastric gavage of 100 mg OVA at PND25. The animals were killed 2 h after the challenge. (B) IgE and IgG levels in amoxicillin- and saline-treated rats (log5 titers). (C and D) Plasma (C) and small intestinal (D) RMCPII levels in rats treated with saline (gray bars) or amoxicillin (white bars), sensitized with OVA, and force-fed saline solution (nonchallenged) or OVA (OVA challenged). (E and F) Light micrographs of the small intestine sampled 2 h after challenge and stained either with chloroacetate esterase activity for mast cell visualization (E) or with hematoxylin and eosin (F) (magnification, ×100) in (a) saline-treated nonchallenged, (b) saline-treated challenged, (c) amoxicillin-treated nonchallenged, and (d) amoxicillin-treated challenged animals. Data are expressed as the means ± standard errors of the means (one experiment; n = 12). Differences were considered significant when P was <0.05 (*).