FIG. 1.

Comparison of the linearities and ranges of detection of WNV RNA extracted from birds and mosquitoes by ABI 6700 workstation and RNeasy methods. Kidney tissue from a WNV-positive crow (A) and a pool of 50 mosquitoes spiked with the head from an infected mosquito (B) were homogenized. Each sample was serially diluted with homogenates prepared from uninfected birds or mosquitoes. RNA was extracted from triplicate aliquots of each dilution by ABI 6700 robot (filled triangles) or RNeasy (empty squares) and assayed by real-time RT-PCR using WNV-specific primers and probe.