TABLE 1.

Comparison of C_T values derived from real-time RT-PCR analysis of RNA samples extracted from bird kidneys naturally infected with WNV

Birda	Results obtained with:				Ratioc
	ABI Prism 6700		RNeasy
	CTb	SD	CT	SD
A	14.97	0.8	16.56	0.7	0.90
B	17.85	0.6	18.45	0.6	0.97
C	14.59	0.6	16.93	0.5	0.86
D	14.71	0.7	16.78	0.6	0.88
E	17.57	0.4	21.95	1.3	0.80
F	30.13	1.5	29.54	5.5	1.02
G	40.00	0.0	40.00	0.0	1.00

^aAmerican crows A through F were infected naturally with WNV; bird G was uninfected.

^bEach C_T value presented here is the average from five separate tissue pieces processed from each bird kidney.

^cRatio was determined by dividing the C_T of the ABI Prism 6700 sample by the C_T of the RNeasy sample.