TABLE 2.

Repeatability (within-assay variability) of WNV RNA purification by ABI Prism 6700 workstation

Birda	CT values obtained with:
	WNV NSIb			WNV envc
	Meand	SD	CVe (%)	Mean	SD	CV (%)
227	16.7	0.51	3.0	15.8	0.39	2.5
228	16.2	0.35	2.2	15.4	0.22	1.5
229	15.9	0.19	1.2	15.3	0.11	0.7
230	15.8	0.22	1.4	15.2	0.13	0.8
231	16.9	0.31	1.8	16.0	0.32	2.0
232	17.1	0.17	1.0	16.1	0.03	0.2
233	16.5	0.90	5.5	15.7	0.62	3.9
234	15.8	0.25	1.6	15.6	0.16	1.0
235	16.4	0.11	0.7	15.4	0.06	0.4
236	16.1	0.05	0.3	15.3	0.03	0.2
302	16.4	0.20	1.2	15.4	0.23	1.5
303	16.9	0.42	2.5	15.4	0.21	1.4
304	16.6	0.53	3.2	15.1	0.13	0.8

^aWNV-infected American crows.

^bWNV NS1-specific primer-probe set.

^cWNV envelope-specific primer-probe set.

^dAverage C_T value, taken from results for four tissue lysate aliquots from each bird, each purified separately by robot.

^eCV, percentage of standard deviation relative to the mean.