TABLE 4.
Comparison of results for WNV RNA purified from infected mosquitoes by using either the 6700 workstation or RNeasy
| WNV-positive mosquito parta (no.) | Results obtained with:
|
|||
|---|---|---|---|---|
| 6700 robot
|
RNeasy
|
|||
| CT | No. of PFUb | CT | No. of PFU | |
| Leg (1) | 31 | 1.4 | 32 | 1.0 |
| 39 | NDc | 33 | 0.3 | |
| 38 | ND | 36 | ND | |
| 40 | ND | 32 | 0.8 | |
| 32 | 1.2 | 40 | ND | |
| Legs (2) | 30 | 3.2 | 28 | 1.1 |
| 30 | 4.4 | 31 | 2.9 | |
| 30 | 3.3 | 32 | 0.9 | |
| Head | 28 | 7.6 | 27 | 16 |
| 26 | 1.8 | 27 | 43 | |
| 27 | 1.6 | 25 | 136 | |
| Abdomen | 27 | 20 | 25 | 79 |
| 24 | 313 | 25 | 101 | |
| Thorax | 26 | 35 | 22 | 593 |
| 25 | 144 | 23 | 387 | |
| 23 | 603 | 23 | 442 | |
a
Pools of 50 uninfected mosquitoes were spiked with the indicated part of an infected mosquito.
b
Relative number of PFU per sample determined by linear regression from WNV standards included in the assay.
c
Not detected; below limit of detection of the assay.