TABLE 5.
Sensitivity of the real-time RT-PCR multiplex assay
| WNV standard (PFU) | Results obtained with:
|
|||
|---|---|---|---|---|
| FAMa
|
VICb
|
|||
| CTc | SD | CT | SD | |
| 8,000 | 17.20 | 0.15 | 17.54 | 0.16 |
| 800 | 20.75 | 0.08 | 21.07 | 0.08 |
| 80 | 24.37 | 0.12 | 24.62 | 0.14 |
| 8 | 27.66 | 0.12 | 28.34 | 0.14 |
| 0.8 | 30.55 | 0.24 | 31.97 | 0.24 |
| 0.08 | 40 | 0 | 40 | 0 |
| 0.008 | 40 | 0 | 40 | 0 |
a
WNV envelope-specific primer-probe set, with probe labeled with FAM fluorescent dye.
b
WNV NS1-specific primer-probe set, with probe labeled with VIC fluorescent dye.
c
Mean of CT values of 10 replicate samples.