TABLE 6.

Reproducibility of results of the real-time RT-PCR multiplex assay

WNV standard (PFU)	Results obtained with:
	FAMa			VICb
	CTc	SD	CVd	CT	SD	CV
8,000	17.26	0.11	0.63	17.31	0.07	0.40
800	20.80	0.10	0.48	21.08	0.15	0.71
80	24.23	0.10	0.41	24.52	0.13	0.53
8	27.58	0.34	1.23	28.05	0.20	0.71

^aWNV envelope-specific primer-probe set, with probe labeled with FAM fluorescent dye.

^bWNV NS1-specific primer-probe set, with probe labeled with VIC fluorescent dye.

^cMean of C_T values of 10 replicate samples

^dCV, percentage of standard deviation relative to the mean.