TABLE 7.
Arbovirus Laboratory assay costs and time commitments
| Task | Sample type(s) | Assay cost per sample ($)a | Time (h) |
|---|---|---|---|
| Recording of specimen information and results and reporting of results | 2.5/50 samples | ||
| Tissue sorting, excision, and homogenization | Birds and mammals | 0.75 | 2/96-well plateb |
| Mosquitoes | 0.50 | 3/96-well plate | |
| Isolation of RNA | |||
| Using RNeasy method | Birds and mammals | 3.25 | 5/96-well plate |
| Mosquitoes | 3.25 | 5/96-well plate | |
| Using ABI 6700 robotc | Birds and mammals | 2.00 | 1/96-well plate |
| Mosquitoes | 2.75 | 1/96-well plate | |
| Real-time RT-PCR (TaqMan) | |||
| Using manual setup | 3.25 | 1/96-well plate | |
| Using ABI 6700 robotc | 3.85 | 0.5/96-well plate | |
| Virus isolation | |||
| Birds and mammals | 5.00 | 2.5/10 samples | |
| Mosquitoes | 1.50 | ||
| IFA (Excising of tissue, cutting of frozen sections, and application of immunocytochemistry) | 7.50 | 5/10 samples |
a
These costs are for specific supplies only and do not reflect costs for equipment, personnel, or general laboratory supplies and services, such as gloves, Kimwipes, underpads, disinfectant, and autoclaving.
b
Each 96-well plate contains 80 samples, 12 WNV standards, and four negative controls.
c
ABI 6700 run time, 85 min for RNA extraction, 45 min for TaqMan setup.