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. 2006 Dec 20;14(2):173–181. doi: 10.1128/CVI.00347-06

FIG. 5.

FIG. 5.

A. Results of IFA test using TPMV-infected Vero E6 cell antigens. As a positive shrew serum control, serum from a shrew experimentally infected with TPMV was used. Sera no. 49 and 69 were positive against TPMV antigen. But sera no. 2 and 79 were negative by the IFA test (data not shown). B. Western blotting analysis of TPMV-positive sera with TPMV antigen. The human positive sera B-3 and B-4 in ELISAs also reacted with TPMV-infected Vero E6 antigen by Western blotting analysis. On the other hand, for shrews, only no. 49 and 69 showed a band at 50 kDa with TPMV antigen, and sera no. 2 and 79 did not. Lanes 1 (B-3) and 2 (B-4) are human positive samples in ELISA; lane 3 (no. 2), lane 4 (no. 49), lane 5 (no. 69), and lane 6 (no. 79) are shrew positive samples in ELISA; lane 7 (no. 2 serum from a shrew experimentally infected with TPMV) is a positive control. Lane 6 (normal shrew serum) is a negative control.