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TroR bound to the tro promoter and blocked transcription. β-galactosidase activity measured in E. coli cells harboring pJAM4 coresident with pKK223–3 (designated by black bar) or pJEP2 (pKK223–3-troR)(designated by gray bar). IPTG was added to induce the expression of TroR and 2′-2 dipyrdil (DIP) to chelate divalent metal ions. The strains were grown in LB (lane 1), LB and IPTG (lane 2), or LB, IPTG, and DIP (lane 3) and assayed as described above.
