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TroR existed as a dimer in the absence of Mn. Immunoblot of purified TroR in chemical crosslinking assay: TroR (lane 1); TroR + 0.2% glutaraldehyde (lane 2); TroR + 0.2% glutaraldehyde + 100 μM MnCl2 (lane 3); TroR + 0.2% gluteraldehyde + 0.5 μg of troPO target DNA (lane 4); and TroR + 0.2% gluteraldehyde + 100 μM MnCl2 + 0.5 μg of troPO target DNA (lane 5). Molecular mass standards are shown on the left.
