TABLE 2.

PCR primers used for gene amplification and multiplex assay

PCR primer use and namea	Primer, DNA sequence
Gene amplification
exoT (1,564-bp fragment)
5′-Primer (position −100)	ExoT5, 5′-CGA CGG CCG CCA ACA GTA AAA-3′
3′-Primer (position 1464)	ExoT3, 5′-AGG TAT CCT TGC CGC CCA TTG-3′
exoU (2,103-bp fragment)
5′-Primer (position −38)	ExoU5, 5′-ATA TTT GCT CCG AAC CCT CG-3′
3′-Primer (position 2065)	ExoU3, 5′-CTG TGC CAG CCA TGT ATC AA-3′
exoY (1,178-bp fragment)
5′-Primer (position −15)	ExoT5, 5′-GCG GGA AAA CGA ACC ATG-3′
3′-Primer (position 1163)	ExoT3, 5′-CGG GCT TTG CCA ACG ACC-3′
Gene detection in multiplex assay
exoS (118-bp fragment)
5′-Primer (position 686)	ExoS-MP5, 5′-GCG AGG TCA GCA GAG TAT CG-3′
3′-Primer (position 804)	ExoS-MP3, 5′-TTC GGC GTC ACT GTG GAT GC-3′
exoT (152-bp fragment)
5′-Primer (position 624)	ExoT-MP5, 5′-AAT CGC CGT CCA ACT GCA TGC G-3′
3′-Primer (position 776)	ExoT-MP5, 5′-TGT TCG CCG AGG TAC TGC TC-3′
exoU (134-bp fragment)
5′-Primer (position 1265)	ExoU-MP5, 5′-CCG TTG TGG TGC CGT TGA AG-3′
3′-Primer (position 1399)	ExoU-MP3, 5′-CCA GAT GTT CAC CGA CTC GC-3′
exoY (289-bp fragment)
5′-Primer (position 755)	ExoY-MP5, 5′-CGG ATT CTA TGG CAG GGA GG-3′
3′-Primer (position 1044)	ExoY-MP3, 5′-GCC CTT GAT GCA CTC GAC CA-3′

^aPosition 1 is at the translational start site.