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. Author manuscript; available in PMC: 2007 Feb 15.
Published in final edited form as: Dev Biol. 2006 Sep 29;302(1):333–344. doi: 10.1016/j.ydbio.2006.09.043

Fig. 4.

Fig. 4

MOs targeting Ci-MRF mRNA reduce muscle gene expression in early gastrula embryos. Representative staining reactions are shown for control embryos assayed for MLC and TnI expression. The majority of MO-treated embryos assayed for MLC expression were negative as shown in the panels. Remaining embryos were significantly down regulated; 21% for MO1640 and 35% for MO4468, or resembled the controls (not shown). Essentially all embryos assayed for TnI expression were negative when treated with either MO. The average number of positive cells after each treatment is indicated below the panels, and includes all cells expressing a given marker irrespective of the strength of the signal detected. The number of embryos examined (n) is shown in parentheses. Scale bar = 50 μm.