. Author manuscript; available in PMC: 2007 Dec 1.

Published in final edited form as: Virology. 2006 Sep 7;356(1-2):165–170. doi: 10.1016/j.virol.2006.08.006

Table 1.

Intracellular neutralization of HIV by transcytosing IgA against internal HIV-1 proteins.^a

	Apical Supernatant		Cell Lysate
MAb (150μg/mL)	Blue Cell #	% Virus Reduction^b	Blue Cell #	% Virus Reduction^b
pIgR⁺ Cells
Control	136 ± 16		1797 ± 194
RT-IgA	84 ± 23	38^c	1143 ± 200	36^c
RT-IgG	133 ± 20	3	1851 ± 197	0
Gag-IgA	81 ± 18	40^c	1176 ± 128	35^c
Gag-IgG	129 ± 17	5	1777 ± 164	1
Irrelevant IgA	131 ± 27	4	1871 ± 357	0
pIgR⁻ Cells
Control	152 ± 12		1943 ± 134
RT-IgA	155 ± 33	0	1872 ± 127	4
Gag-IgA	159 ± 32	0	1933 ± 137	1

Polarized monolayers of pIgR⁺ or pIgR⁻ Vero C1008 cells were transfected with HIV proviral DNA for 4 h and then exposed to MAbs basolaterally for 18 h. HIV levels in apical supernatants and cell lysates 30 h post transfection were determined by infecting target HeLa cells (“blue cell” assay). Data are means from eight data points ± SEM pooled from four experiments.

Compared to the no antibody control.

Result significantly different (p<0.005) from the no antibody control according to a two-tailed t-test.