Table 2.
IgA antibody concentration dependence of intracellular virus neutralization and comparison to conditioned media a
| Apical Supernatant
|
Cell Lysate
|
|||
|---|---|---|---|---|
| IgA MAb (μg/mL) | Blue Cell # | % Virus Reductionb | Blue Cell # | % Virus Reductionb |
| Control | 156 ± 26 | 1781 ± 123 | ||
| Anti-RT (150) | 96 ± 21 | 39 c | 1075 ± 125 | 40c |
| Anti-RT (50) | 115 ± 14 | 26c | 1336 ± 252 | 25c |
| Anti-RT (20) | 126 ± 34 | 19c | 1674 ± 133 | 6c |
| Control | 145 ± 21 | 1814 ± 76 | ||
| Anti-Gag (150) | 100 ± 16 | 31c | 1330 ± 158 | 27c |
| Anti-Gag (50) | 114 ± 16 | 21c | 1489 ± 122 | 18c |
| Anti-Gag (20) | 140 ± 23 | 4 | 1808 ± 202 | 1 |
| Control | 137 ± 15 | 1776 ± 43 | ||
| Anti-RT (150) | 82 ± 14 | 40c | 1086 ± 99 | 39c |
| Anti-Gag (150) | 80 ± 20 | 42c | 1032 ± 138 | 42c |
| C.M.1- Anti-RTd | 125 ± 33 | 9 | 1707 ± 114 | 4 |
| C.M.1- Anti-GAGd | 125 ± 20 | 9 | 1774 ± 124 | 2 |
| C.M.2- Anti-RTe | 1717 ± 159 | 3 | ||
| C.M.2- Anti-GAGe | 1774 ± 124 | 0 | ||
a
Experiments as in Table 1 except that only pIgR+ epithelial cells were used. In some cases conditioned medium (C.M.) was added to the apical chamber or cell lysate without MAb having been added to the basolateral surface. Data are means from eight data points ± SEM pooled from four experiments.
b
Compared to no antibody control.
c
Result significantly different (p≤0.03) from the no antibody control.
d
C.M. 1 - apical supernatant with transcytosed IgA (see text).
e
C.M. 2 - IgA-transcytosing cell lysate (see text).