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. 2006 Dec 8;6(2):182–197. doi: 10.1128/EC.00364-06

FIG. 2.

FIG. 2.

Cells lacking AgCDC23 and AgDOC1 are inviable and arrest in mitosis. (A) Spores from wild-type (WT) A. gossypii cells were grown for 15 h at 30°C and processed for antitubulin immunofluorescence and DNA staining. (B) Brightfield images depicting “late lethal” arrest point as small mycelia from Agdoc1Δ and Agcdc23Δ mutants. (C and D) Spores from Agcdc23Δ heterokaryons (RNSN01) and Agdoc1Δ heterokaryons (RNSN02), respectively, were grown under selection for 15 h at 30°C and processed for antitubulin immunofluorescence and DNA staining. Arrows point to examples of mitotic nuclei. Bar, 10 μm.