FIG. 5.

AgPds1p is present in nuclei but diminishes in anaphase. Spores from a strain expressing AgPds1p-HA (NSG09) were grown under selective conditions and then processed for immunofluorescence. (A) Segment of a branched hypha showing four nonmitotic nuclei and one anaphase nucleus (arrow). Only the nonmitotic nuclei contain AgPds1p-HA. (B) AgPds1p-HA localization in different nuclear cycle stages showing its presence in nuclei with one or two spindle pole bodies and in metaphase (Meta) nuclei but not in anaphase (Ana) nuclei. (C) Hyphae were released from a nocodazole block to follow AgPds1p-HA through anaphase in a large number of nuclei. Weak AgPds1p-HA signals are observed only in maximally extended spindles (bottom panels). Bar, 5 μm.