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APC activators AgCdh1p and AgCdc20p localize to all nuclei independent of the nuclear cycle stage. (A) Immunostained hyphae expressing N-terminally GFP-labeled AgCdh1p (NSG12). (B) Immunostained hyphae expressing N-terminally GFP-labeled AgCdc20p (NSG13). All nuclei, including anaphase nuclei (arrows), show anti-GFP immunofluorescence. The nuclei of nontransformed hyphae were not stained (see Fig. S1 in the supplemental material). Bar, 10 μm.
