TABLE 2.
Plasmids used in this study
| Plasmid | Vector | Important sequence features | Source or reference |
|---|---|---|---|
| pGEN3 | GEN3 | 52 | |
| pRS416 | pUC19 | ScURA3 CEN ARS | 46 |
| pRS415 | pUC19 | ScLEU2 CEN ARS | 46 |
| pAGT146 | pUC19 | 13myc-ScURA3term-GEN3 | A. Kaufmann |
| pG3-9Myc | pUC19 | 9myc-KlIPP1term-GEN3 | A. Kaufmann |
| pK16Bni-GFP | pUC19 | GEN3-promAgBNI1-GFP | M. Köhli |
| pUC19NATP | pUC19 | CloNAT | D. Hoepfner |
| pAGT145 | pUC19 | GEN3-6HA | A. Kaufmann |
| pAG7019 | pRS416 | AgCDH1 | 12 |
| pAG14820 | pRS416 | AgPDS1 | 12 |
| pAG6955 | pRS416 | AgCDC23 | 12 |
| pAG1393 | pRS416 | AgDOC1 | 12 |
| pNS01 | pRS416 | AgCDC20 | This study |
| pGVS1 | pRS415 | AgCDC16 | This study |
| pGVS2 | pRS415 | AgCDC16-13myc-GEN3 | This study |
| pGVS3 | pAG1393 | AgDOC1-13myc-GEN3 | This study |
| pGVS4 | pAG6955 | AgCDC23-13myc-GEN3 | This study |
| pNS07 | pAG14820 | Agpds1Δdb-6HA-GEN3 | This study |
| pNS09 | pAG14820 | Agpds1Δdb | This study |
| pNS015 | pRS416 | GEN3-prom-GFP-AgCDH1 | This study |
| pNS016 | pRS416 | GEN3-prom-GFP-AgCDC20 | This study |