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mpt5Δ mutants display a short chronological life span, a defect suppressed by overexpression of PKC1. Wild-type (WT; JVG161) and mpt5Δ (JVG2110) cells were transformed with a (A) vector or (B) pPKC1 (pDL289). The transformants were grown to stationary phase in synthetic dropout medium at 30°C. Numbers of CFU were determined as a function of time after saturation by plating dilutions onto YPD plates.
