FIG. 3.

Overexpression of MPT5 suppresses the growth defect of slg1Δ mutants but not mutants affecting downstream signaling in the CWI pathway. slg1Δ/slg1Δ (JVG1081) mutants were transformed with a vector (YEp24), pMPT5 (pG19), or pSLG1 (pJO36). Transformants were selected at 25°C, streaked onto fresh plates, and incubated at 25°C or 30°C for 3 days prior to being photographed. Similar experiments were performed with other mutant strains containing either pkc1-1, bck1Δ, or slt2Δ (data not shown). The results from all these analyses are tabulated here, where “+” indicates suppression of the temperature-sensitive growth defect of each strain by overexpression of MPT5 (pG19) and “−” indicates no such suppression.