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PMT4 disruption results in abnormal budding morphology and defective cell separation. (A) Phase-contrast and fluorescent microscopic observation of septum formation and morphology in Alexa Fluor 488-conjugated WGA-stained C. neoformans yeast cells. (B) India ink-stained C. neoformans yeast cells after 4 days of capsule induction in Dulbecco modified Eagle medium. Magnification, ×1,000.
