FIG. 5.
Effect of PMT4 disruption on sensitivity to elevated temperature, SDS, and amphotericin B. (A) C. neoformans strains H99 (wild type), GMO1 (pmt4), and GMO3 (pmt4 plus PMT4) were grown to mid-log phase, normalized to 2 × 107 CFU/ml, serially diluted, and spotted onto YPD medium (or YPD plus 0.025% SDS) and incubated for 2 days at 30°C, 37°C, or 39°C. (B) C. neoformans strains H99 (wild type), GMO1 (pmt4), and GMO3 (pmt4 plus PMT4) (5 × 105 CFU) were plated on 0.5× YPD plates and overlaid with amphotericin (AP) E-test strips for 3 days at 39°C. AP MICs (in μg/ml): H99, 0.25; GMO1, 0.125; GMO3, 0.25.