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Immunolocalization of T. cruzi TcSofI in epimastigote forms. (A) Phase-contrast microscopy. (B) Propidium iodide staining. (C) Immunofluorescence analysis showing dispersed labeling of the nucleus. (D) Ultrastructural immunocytochemistry confirmed the labeling in the nucleus, with gold particles distributed throughout the nucleus. (A, B, and C) Arrowheads indicate the kinetoplast structure, and arrows indicate the nucleus. (A, B, and C) Bar, 10 μm. (D) Bar, 0.25 μm.
