FIG. 5.

Polysome profiles of epimastigote (A) and metacyclic trypomastigote (E) forms. The positions of the 40S and 60S subunits, the 80S ribosome monomer, and polysomes are indicated in the sucrose density gradient. Fractions were collected and pooled (RF, ribosome free; S/M, subunits and monomers; P, polysomes), and RNA was purified. Northern blots of epimastigote fractions (B and C) and metacyclic trypomastigote fractions (F and G) analyzed with the radioactively labeled TcSof1 gene probe are shown. (D) The membrane shown in panel C was stripped and reprobed with an H2B histone gene probe.