FIG. 6.

Polysome profile of metacyclic trypomastigote forms after puromycin treatment (A). Fractions were collected and pooled (RF, ribosome free; S/M, subunits and monomers; P, polysomes), and RNA was purified (B). Northern blot analysis of metacyclic trypomastigote fractions (C) hybridized with the radioactively labeled TcSof1 gene probe is shown. SDS-PAGE analysis of immunoprecipitated labeled proteins from epimastigote (E) and metacyclic trypomastigote (M) soluble fractions with the anti-TcSof1 (D) and anti-PEPCK (E) sera is shown. Proteins were separated in a 10% SDS-PAGE gel, and the gel was dried and exposed to X-ray films for 5 days.