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. 2006 Dec 22;6(2):134–142. doi: 10.1128/EC.00290-06

FIG. 4.

FIG. 4.

Gcn4-dependent activation of QDR2 transcription when the auxotrophic strain BY4741 enters stationary phase due to leucine limitation. (A) Relative levels of QDR2 mRNA in S. cerevisiae BY4741 cells during growth in MM4 liquid medium. Cell samples were harvested at the times indicated by the letters in the figure. Relative ratios of QDR2 mRNA to ACT1 mRNA were obtained by densitometry of the autoradiograms. The basal QDR2 mRNA relative value of exponential cells at an OD600 of 0.3 ± 0.05 was set as 1. (B) Relative levels of QDR2 mRNA in S. cerevisiae BY4741 (▪) and BY4741 Δgcn4 (□) cells during growth in MM4 medium. Cell samples were harvested at different times of cultivation, as indicated by the black arrows and the letters, during exponential growth in early stationary phase and 1 hour following the addition of fresh leucine to starved cells, as indicated by the white arrows. The growth curves in medium without extra leucine supplementation are represented by dotted lines. Relative ratios of QDR2 mRNA to ACT1 mRNA were obtained by densitometry of the autoradiograms. The basal QDR2 mRNA relative value of exponential cells at an OD600 of 0.3 ± 0.05 was set as 1. Values are representative of two independent experiments.