TABLE 1.
Concentrations of amino acids in Saccharomyces cerevisiae BY4741 and BY4741 Δqdr2 cellsa
| Amino acid | Amino acid concn (μmol/g of cells [dry wt]) in:
|
|||
|---|---|---|---|---|
| Exponential phase
|
Early stationary phase
|
|||
| WT cells | Δqdr2 cells | WT cells | Δqdr2 cells | |
| Alanine | 37.0 | 49.0 | 123.9 | 200.1 |
| Arginine | 32.5 | 50.4 | 52.4 | 95.8 |
| Asparagine | 11.8 | 18.4 | 21.2 | 29.6 |
| Aspartate | 9.3 | 18.0 | 22.5 | 25.9 |
| Cysteine | 15.1 | 20.2 | 9.6 | 15.4 |
| Glutamine | 5.7 | 9.7 | 21.2 | 41.8 |
| Glycine | 4.3 | 6.5 | 8.1 | 9.2 |
| Isoleucine | 1.3 | 2.2 | 2.6 | 4.9 |
| Lysine | 11.3 | 17.1 | 29.8 | 31.0 |
| Phenylalanine | 1.7 | 1.6 | 2.7 | 4.9 |
| Serine | 4.4 | 7.9 | 11.5 | 12.3 |
| Threonine | 4.3 | 7.2 | 9.4 | 19.7 |
| Tryptophan | 11.3 | 11.6 | 5.7 | 8.4 |
| Tyrosine | 0.8 | 1.8 | 5.4 | 15.0 |
| Valine | 6.1 | 9.1 | 9.8 | 18.8 |
| Histidine* | 11.4 | 8.9 | 29.2 | 41.9 |
| Leucine* | 2.5 | 2.2 | 1.3 | 2.5 |
| Methionine* | 1.0 | 1.2 | 6.5 | 10.7 |
a
Cells were harvested in mid-exponential growth phase or when the culture entered the stationary phase of growth (OD600 of 2) due to leucine limitation. The amino acids corresponding to BY4741 auxotrophies are indicated by an asterisk. WT, wild type.