FIG. 1.
Real-time PCR assay for evaluation of HHV-8 susceptibility to ganciclovir. (A) Amplification plots showing threshold cycle (Ct) values obtained for the HHV-8 ORF26 gene in presence of serial concentrations (from 0 to 32 μM) of ganciclovir. (B) Standard curve of the real-time PCR assay generated using serial 10-fold dilutions of an external HHV-8 standard and from which the Ct values of the different drug concentrations were interpolated. (C) Calculation of the ganciclovir IC50 value based on relative viral load for each drug concentration compared to induced BCBL-1 cells with no drug.