FIG. 3.

The effect of broad-spectrum cyclooxygenase inhibitors on cryptococcal prostaglandin production. Lysates from C. neoformans (C.neo) cells were incubated with or without AA with or without inhibitor for 2 h at 37°C. Enzymatic involvement was demonstrated using boiled lysate. Background, i.e., nonenzymatic, conversion of AA was measured for a sample with AA alone and subtracted from all values. (A) Total prostaglandin production was measured using a prostaglandin-screening enzyme immunoassay (*, P value of <0.05 by unpaired t test). (B) Inhibitory effect of cyclooxygenase inhibitors expressed as percent inhibition (where the lysate-plus-AA sample value was set at 100%) (*, P value of <0.001 by one-way analysis of variance with Bonferroni's posttest). Statistically, results for the boiled lysates are not different from those for buffer alone.