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Temperature and carbon source affect alternative carbon utilization. The C. albicans wild-type (SC5314), fbp1Δ (MRC14), fbp1Δ + FBP1 (MRC15), icl1Δ (MRC10), icl1Δ + ICL1 (MRC11), fox2Δ (MRC6), and fox2Δ + FOX2 (MRC5) strains were grown to mid-log phase and serially diluted, 1:5, in a 96-well plate. The dilutions were spotted using a multiprong replicator onto minimal YNB media containing the indicated carbon source at 2%.
