Figure 1.

ERβ and ERα are expressed in early postnatal DRG. DRG sections were prepared from P3–P4 rats and incubated with an antiserum against ERβ (A–D) and ERα (E–H) as described in Materials and Methods. (A and E) Control without primary antibody. (B and F) Low magnification. (C, D, G–H) High magnification. Nuclear staining of DRG neurons is clearly visible with some staining of the cytoplasm for ERβ. (Bar = 30 μm.)