Figure 2.

(A) Effect of clotrimazole and ketoconazole on T cell proliferation. Cells were incubated for 5 days in culture medium with PPD and the IK channel blockers (10 μM clotrimazole or 10 μM ketoconazole), which were added 30 min before the addition of PPD. [³H]Thymidine (1 mCi) incorporation was then measured in triplicate wells. The bars represents 12 independent experiments ± SE; control = 26 ± 12 × 10³ cpm per well; clotrimazole = 12 ± 6 × 10³ cpm per well; ketoconazole = 20 ± 10 × 10³ cpm per well. ∗, P ≤ 0.008 vs. control. (B) Effect of nitrendipine, clotrimazole, and charybdotoxin on T cell proliferation. Cells were incubated for 5 days in culture medium with Candida albicans and the IK channel blockers (10 μM nitrendipine, 100 nM charybdotoxin, or 10 μM clotrimazole), which were added 30 min before the addition of Candida albicans. [³H]Thymidine (1 mCi) incorporation was then measured in triplicate wells. The bars represents 9 independent experiments ± SE for control, and 3 independent experiments in the presence of nitrendipine, clotrimazole, and charybdotoxin, respectively. ∗, P ≤ 0.05 vs. control; ∗∗, P ≤ 0.01 vs. control.