Figure 3.

(Upper) Dose–response relations of nitrendipine, clotrimazole, and charybdotoxin on T cell proliferation. Cells were incubated for 5 days in culture medium with PPD. The IK channel blockers, in the concentrations indicated, were added 30 min before the addition of antigen. [³H]Thymidine (1 mCi) incorporation was then measured in triplicate wells. The bars represents nine independent experiments ± SE for control, and three independent experiments in the presence of nitrendipine, clotrimazole, or charybdotoxin, respectively. ∗, P ≤ 0.05 vs. control. Six other experiments using Candida albicans or tetanus toxin as the antigen challenge gave similar results. (Lower) Donor variability of the clotrimazole effect on T cell proliferation. PHA, phytohemagglutinin. Cells from three different donors were incubated for 5 days in culture medium with mitogen (ConA or PHA) or antigen (tetanus toxin or PPD) and 10 μM clotrimazole, which was added 30 min before the addition of Candida albicans. [³H]Thymidine (1 mCi) incorporation was then measured in triplicate wells. The bars represents three independent experiments ± SE.