Fig. 2.

PCR and Southern blot results of a selection of eight strains of M. mycoides subsp. mycoides SC for investigation of the 24-kb repeats. PCR was performed with 50 ng of genomic DNA using oligonucleotide primers: (A) MmmSC_23760fwd and MmmSC_1rev to obtain fragment A of Fig. 1A; (B) MmmSC_rpoC_fwd and MmmSC_MSC1011rev to obtain fragment B of Fig. 1A; and (C) MmmSC_23760fwd and MmmSC_MSC1061rev to obtain fragment C of Fig. 1A. (D) Southern blot hybridization of ClaI-, SacI-, and MluNI-digested DNA hybridized to the asnA probe. Std, molecular mass standards: 23.1, 9.4, 6.6, 4.4, 2.3, and 2.0 kb.