Table 3.

TaqMan analysis for determination of copies of the lppQ gene

Strain	CtlppQa	CtglpOa	ΔCtb	ΔΔCtc	2(1+ΔΔCt)d	lppQ copies
PG1	16.69	18.50	1.81	0.00	2.00	2
C305	17.00	17.86	0.86	− 0.95	1.04	1
PO 2	16.33	17.16	0.83	− 0.98	1.01	1
2022	16.51	17.25	0.74	− 1.07	0.95	1
L2	16.46	17.23	0.77	− 1.04	0.97	1
Afadé	16.98	17.90	0.92	− 0.89	1.08	1
Fatick	16.28	16.99	0.71	− 1.10	0.93	1
B17	16.33	17.24	0.91	− 0.90	1.07	1
95014	16.66	17.43	0.77	− 1.04	0.97	1
T1/44	16.06	16.88	0.82	− 0.99	1.01	1
T1/Sr50	16.84	17.71	0.87	− 0.94	1.04	1
Gladysdale	16.88	17.76	0.88	− 0.93	1.05	1
R575	16.16	16.89	0.73	− 1.08	0.95	1
DVZ	16.19	16.93	0.74	− 1.07	0.95	1
V5	16.41	17.14	0.73	− 1.08	0.95	1

^aThe values are the means of two or three independent measurements.

^bCt_glpO–Ct_lppQ (normalization of endogenous control).

^cΔCt_strain–ΔCt_PG1 (normalization to PG1).

^dRatio between copies of lppQ and copies of glpO.