Table 2 Ninety five ESTs that cluster patients with Crohn's disease and those with SpA and chronic gut inflammation.
| Accession | Unigene | Symbol | Gene description | Cytogenetic location | Expression in CD/SpA chronic | Marker | Score | Ref. |
|---|---|---|---|---|---|---|---|---|
| H81904 | Hs.14779 | ACAS2 | Acetyl‐coenzyme A synthetase 2 (ADP forming) | 20q11.22 | ↓ | |||
| AI758270 | ↓ | |||||||
| R20596 | Hs.101359 | C6orf32 | Chromosome 6 open reading frame 32 | 6p22.3–p21.32 | D6S2439 | LOD 2.6 | Barmada et al25 | |
| AI349525 | Hs.121692 | FLJ34790 | Hypothetical protein FLJ34790 | 17p13.1 | ↓ | |||
| AI445844 | Hs.444422 | PDK3 | Pyruvate dehydrogenase kinase, 3 | Xq22 | ↓ | DXS1226‐DXS1214 | LOD 2.0 | van Heel et al26 |
| H56656 | Hs.282958 | FLJ13611 | 5q12.3 | ↓ | ||||
| N22829 | Hs.299055 | GDI2 | GDP dissociation inhibitor 2 | 10p15 | ↓ | |||
| AI274555 | Hs.170568 | TATDN1 | TatD DNase domain containing | 8q24.13 | D8S198 | NPL 2.47 | Vermeire et al27 | |
| H61436 | Hs.184668 | SNX24 | Sorting nexin 24 | 5q23.2 | ||||
| H15751 | Hs.30634 | PARP16 | Poly (ADP‐ribose) polymerase family, member 16 | 15q22.31 | ↓ | |||
| H87107 | Hs.216354 | RNF5 | Ring finger protein 5 | 6p21.3 | ↓ | D6S1281‐D6S1019 | LOD 2.3 | Rioux et al28 |
| AI680066 | ||||||||
| AI191504 | Hs.510373 | AK7 | Adenylate kinase 7 | 14q32.2 | ↓ | |||
| R17390 | Hs.80132 | SNX15 | Sorting nexin 15 | 11q12 | ||||
| AA812701 | Hs.343631 | LOC340178 | Hypothetical protein LOC340178 | 6q27 | ||||
| AA825971 | Hs.209561 | KIAA1715 | 2q31 | |||||
| AA291593 | Hs.3402 | SLC39A11 | Solute carrier family 39 (metal ion transporter) member 11 | 17q24.3–q25.1 | ||||
| Hs.118836 | MB | Myoglobin | 22q13.1 | D22S689 | LOD 1.5 | Barmada et al25 | ||
| AA420968 | Hs.98323 | C6orf112 | Chromosome 6 open reading frame 112 | 6q21 | ||||
| R08643 | Hs.350268 | IRF2BP2 | Interferon regulatory factor 2 binding protein 2 | 1q42.3 | ||||
| T83584 | Hs.115945 | MANBA | Mannosidase, βA, lysosomal | 4q22–q25 | D4S406 | NPL 2.17 | Vermeire et al27 | |
| AA481482 | Hs.67928 | ELF3 | E74‐like factor 3 (ets domain transcription factor, epithelial specific ) | 1q32.2 | ||||
| T78477 | Hs.206770 | ZNF297 | Zinc finger protein 297 | 6p21.3 | D6S121‐D6S1019 | LOD 2.3 | Rioux et al28 | |
| H27674 | Hs.238465 | DAB2IP | DAB2‐interacting protein | 9q33.1–q33.3 | ||||
| R13194 | Hs.32935 | BRF1 | BRF1 homologue, subunit of RNA polymerase III transcription initiation factor IIIB | 14q | ||||
| R98758 | Hs.380474 | FLJ32731 | Hypothetical protein FLJ32731 | 8p11.1 | ||||
| H96900 | Hs.356467 | MGC2747 | MGC2747 | 19p13.11 | ||||
| N62199 | Hs.375193 | KIF1B | Kinesin family member 1B | 1p36.2 | ↓ | D1S1597 | LOD 3.01 | Cho et al29 |
| R51052 | Hs.296169 | SPHAR | S‐phase response (cyclin‐related) | 1q42.11–q42.3 | ↓ | |||
| AI286348 | Hs.443728 | SH3RF2 | SH3 domain containing ring finger 2 | 5q32 | ||||
| R17478 | Hs.497159 | PIG13 | Proliferation‐inducing protein 13 | 1q25 | ||||
| AI916359 | Hs.205842 | ATP2C2 | Secretory pathway calcium ATPase 2 | 16q24.1 | ||||
| H19034 | Hs.5300 | BLCAP | Bladder cancer‐associated protein | 20q11.2–q12 | ||||
| N39050 | Hs.93825 | FLJ39784 fis | Homo sapiens cDNA FLJ39784 fis, clone SPLEN2002314 | |||||
| R13544 | Hs.7540 | FBXL3A | F‐box and leucine‐rich repeat protein 3A | 13q22 | ↓ | |||
| AI286163 | Hs.122428 | ↓ | ||||||
| R07321 | Hs.464137 | ACOX1 | Acyl‐coenzyme A oxidase 1, palmitoyl | 17q24–17q25 | ↓ | |||
| R12168 | Hs.96870 | STAU2 | Staufen, RNA‐binding protein, homologue 2 (Drosophila) | 8q13–q21.1 | ↓ | |||
| H85472 | Hs.75789 | NDRG1 | N‐myc downstream‐regulated gene 1 | 8q24.3 | ↓ | |||
| AA513663 | ↓ | |||||||
| R97820 | Hs.325568 | ZC3HAV1 | Zinc finger CCCH type, antiviral 1 | 7q34 | ↓ | |||
| N62837 | Hs.48647 | ILT7 | Leucocyte Ig‐like receptor, subfamily A (without TM domain), 4 | 19q13.4 | ↓ | |||
| AA019615 | Hs.145469 | UCHL5 | Ubiquitin carboxyl‐terminal hydrolase L5 | 1q32 | ↓ | |||
| AI809092 | ↓ | |||||||
| AA758064 | Hs.401400 | |||||||
| AI219353 | Hs.89387 | CASC2 | Cancer susceptibility candidate 2 | 10q26.11 | ↓ | |||
| AI698801 | Hs.202187 | |||||||
| R12632 | Hs.109706 | HN1 | Haematological and neurological expressed 1 | 17q25.1 | ||||
| AA468418 | ||||||||
| Hs.28777 | HIST1H2AC | Histone 1, H2ac | 6p21.3 | D6S2439 | LOD 2.6 | Barmada et al25 | ||
| H23734 | Hs.522584 | TMSB4X | Thymosin, β4, X linked | Xq21.3–q22 | ||||
| H75688 | Hs.197015 | SNX7 | Sorting nexin 7 | 1p21.3 | ||||
| N63669 | Hs.81541 | TRIO | Triple functional domain (PTPRF interacting) | 5p15.1–p14 | ||||
| AI819016 | ↑ | |||||||
| AI831827 | Hs.211194 | ARFGEF2 | ADP‐ribosylation factor guanine nucleotide exchange factor 2 | 20q13.13 | ||||
| AI923299 | Hs.213456 | LOC158730 | Hypothetical LOC158730 | Xp21.1 | ↑ | |||
| AI286257 | Hs.124553 | ZNF263 | Zinc finger protein 263 | 16p13.3 | ||||
| AI863417 | Hs.208726 | RUNX1 | Runt‐related transcription factor 1 (acute myeloid leukaemia 1; aml1 oncogene) | 21q22.3 | ↑ | |||
| AI921132 | ||||||||
| AI274438 | ↑ | |||||||
| AI289775 | Hs.387091 | EST, weakly similar to 0412263A Ig G2 pFc' PIG Gm | ↑ | |||||
| AI345173 | ||||||||
| AI928384 | ||||||||
| AA490519 | Hs.16165 | CDNA clone IMAGE:5286005 | ||||||
| AI696951 | Hs.355460 | CDNA: FLJ21763 fis | ||||||
| AI339536 | Hs.348436 | Homo sapiens, clone IMAGE:5286812, mRNA | ||||||
| AI245190 | ||||||||
| AI261428 | Hs.145523 | |||||||
| AI809310 | Hs.210385 | HERC1 | Hect domain and RCC1 (CHC1)‐like domain 1 | 15q22 | ||||
| AI831536 | Hs.413608 | IL12RB2 | Interleukin 12 receptor, β2 | 1p31.3–p31.2 | ||||
| T79944 | ↑ | |||||||
| AA480677 | Hs.161954 | Homo sapiens, clone IMAGE:4865533 | ↑ | |||||
| AA009461 | Hs.172210 | MUF1 | MUF1 protein | 1p34.1 | ↑ | D1S197 | NPL 2.07 | Vermeire et al27 |
| R61783 | Hs.5097 | SYNGR2 | Synaptogyrin 2 | 17q25.3 | ↑ | |||
| AI282992 | Hs.2704 | GPX2 | Glutathione peroxidase 2 (gastrointestinal) | 14q24.1 | ↑ | |||
| W65310 | ||||||||
| AA588676 | Hs.162575 | PDLIM3 | PDZ and LIM domain 3 | 10q22.3–q23.2 | ||||
| AI811147 | ↑ | |||||||
| AI357718 | ||||||||
| N58523 | Hs.137274 | |||||||
| W74496 | ↑ | |||||||
| AI352320 | ↑ | |||||||
| AI280708 | Hs.7155 | LOC129607 | Thymidylate kinase family LPS‐inducible member | 2p25.2 | ||||
| AI815599 | Hs.90744 | PSMD11 | Proteasome (prosome, macropain) 26S subunit, non‐ATPase, 11 | 17q11.2 | ||||
| AI718161 | ||||||||
| N98921 | Hs.85844 | TPM3 | Tropomyosin 3 | 1q21.2 | D1S305 | NPL 2.97 | Vermeire et al27 | |
| AI367201 | Hs.127496 | HSPC135 | HSPC135 protein | 3q13.2 | ||||
| AA425545 | Hs.104613 | RP42 | RP42 homologue | 3q26.3 | ||||
| H44213 | Hs.374986 | Hypothetical gene supported by BC031266 | ||||||
| N58936 | Hs.339283 | NCOA7 | Nuclear receptor coactivator 7 | 6q22.32 | ||||
| N90208 | Hs.344478 | FLJ32440 | Hypothetical protein FLJ32440 | 8q24.13 | D8S198 | NPL 2.47 | Vermeire et al27 | |
| AA427403 | Hs.366 | IFITM1 | Interferon‐induced transmembrane protein 1 (9‐27) | 11p15.5 | ||||
| R25648 | Hs.23920 | PI4K2B | Phosphatidylinositol 4‐kinase type IIβ | 4p15.2 | ||||
| N93265 | Hs.75428 | SOD1 | Superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) | 21q22.11 | ||||
| H99865 | Hs.91954 | NR2F2 | Nuclear receptor subfamily 2, group F, member 2 | 15q26 | ||||
BRF1, butyrate response factor 1; CD, Crohn's disease; Zo1, zonula occludens‐1; DAB2, disabled homolog 2; Dlg, discs‐large protein; EST, expressed sequence tag; Ig, immunoglobulin; LIM, Lin‐11, ls1‐1, Mec‐3; LOD, logarithm of the ratio of the odds that two loci are linked; LPS, lipopolysaccharide; NPL, non‐parametric LOD score; PSD, postsynaptic density protein; SpA, spondyloarthritis.
Genes with aberrant expression in patients with Crohn's disease and in those with SpA and chronic gut inflammation (↓ down regulated and ↑ up regulated as compared with the controls and those with SpA; p<0.05).
Genetic markers are cited for genes that are located within or near (5 cM) one of the Crohn's disease locus, together with the score supporting linkage of the loci.