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. 2007 Feb 12;402(Pt 2):261–269. doi: 10.1042/BJ20061427

Figure 7. Lck is required for full Cr(VI)-stimulated STAT3 phosphorylation.

Figure 7

(A) BEAS 2B cells at 70–80% confluence were transiently transfected with plasmids containing either pRK5 or dominant-negative (dn) JAK2. After 24 h, cells were exposed to Cr(VI) (5 μM) for 72 h or to 0.01 μg/ml of IL-6 for 30 min prior to the end of the experiment. Western-blot analysis was performed on 30 μg of total cell lysate. (B) BEAS 2B cells were transiently transfected with various amounts of siRNA specific to human Lck. Total cell lysates were obtained after 96 h and Lck and β-actin protein was measured by Western-blot analysis. (C) BEAS 2B cells were transiently transfected with 200 nM siRNA specific to human Lck or non-specific, random siRNA (NS). After 72 h, cells were exposed to Cr(VI) (5 μM) for an additional 72 h. IL-6 (0.01 μg/ml) was added for 30 min prior to the end of the experiment. Nuclear proteins were extracted at the end of the experiments and probed for pY-STAT3 and β-actin by Western-blot analysis. The results are representative of three separate experiments.