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Sea urchin egg homogenates were loaded with 1–10 μM Acridine Orange (A,C and D) or 10 μM Lysosensor™ Green DND-189 (B). Homogenates were treated with 1 μM NAADP, 1 μM β-NAD+, 1 μM NADP, 100 μM GPN, 10 mM NH4Cl or 2 μM nigericin (Nig) where indicated. Traces are representative of at least three preparations.
