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. 2007 Feb 12;402(Pt 2):367–375. doi: 10.1042/BJ20061072

Figure 1. IEX-1 inhibits the activity of the 26 S proteasome.

Figure 1

(a) Tet-293-mock and Tet-293-IEX-1 cells (clones a3 and a10) treated with or without tetracycline (16 h, 1 μM), or (b) 293-babe, 293-Ri2/10 and 293-Ri2/9 cells were submitted to a fluorimetric 26 S proteasome assay. For determination of residual non-proteasomal activity accounting for AMC formation, the specific 26 S proteasome inhibitor MG-132 was added in parallel. Results are expressed as n-fold AMC formation in comparison with control cells without Suc-LLVY-AMC and express the means±S.D. for five independent experiments performed in duplicate and adjusted to equal cell numbers.