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. 2007 Feb 12;402(Pt 2):367–375. doi: 10.1042/BJ20061072

Figure 4. The decreasing effect of IEX-1, but not of etoposide, on the protein levels of S1 and S5a is caspase-independent.

Figure 4

(a) Tet-293-mock and Tet-293-IEX-1 cells (clone a3) pretreated with or without tetracycline (8 h, 1 μM), or (b) 293-babe, 293-Ri2/10 and 293-Ri2/9 cells were subjected to treatment with or without etoposide (10 μM) for 24 h. Then, cell lysates were submitted to Western blot analysis detecting the proteasomal proteins S1 and S5a or, as the control, Hsp90. (c) Tet-293-IEX-1 cells (clone a3) pretreated with or without tetracycline (8 h, 1 μM), or (d) 293-babe and 293-Ri2/10 cells were subjected to treatment with etoposide (10 μM) for 24 h or not, either in the absence (left four lanes) or presence (right four lanes) of Z-VAD-FMK. Then, cell lysates were submitted to Western blot analysis detecting the proteasomal proteins S1 and S5a or, as the control, Hsp90. In all panels, one representative result from three independent experiments is shown.