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. 2007 Feb 12;402(Pt 2):367–375. doi: 10.1042/BJ20061072

Figure 5. IEX-1 suppresses gene expression of S1 and S5a on the transcriptional level.

Figure 5

Total RNA from (a) Tet-293-mock and Tet-293-IEX-1 cells (clones a3 and a10) pretreated with tetracycline (16 h, 1 μM) or from (b) 293-babe, 293-Ri2/9 and 293-Ri2/10 cells was reverse-transcribed and then submitted to semi-quantitative real-time PCR using specific primers for S5a, S6′, S1, C2 and, as the control, β-actin. The amount of mRNA normalized to β-actin is shown and expressed as means±S.D. (n=5); asterisk indicates statistically significant (P<0.05).