Figure 3. Tankyrase knockdown impairs glucose uptake reversibly and dose-dependently.

Adipocytes were electroporated on day 6 with a tankyrase siRNA (T1A or T1B), a point-mutation control (Pmt) or buffer alone (Buff). (A) Extracts prepared on day 8 (lanes 1–6) or day 9 (lanes 7–9) were immunoblotted for tankyrase (upper panels) and tankyrase-2 (lower panels). (B) [³H]Deoxy-D-glucose uptake in the basal state and after insulin stimulation (20 nM for 20 min) was determined on day 8 (left-hand panel) or day 9 (right-hand panel) as in Figure 1(B). The results shown are representative of four (day 8) or two (day 9) experiments. TNKS-1, tankyrase-1; TNKS-2, tankyrase-2.